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Gel Electrophoresis Buffers and Diluents

Buffer and diluent solutions to prepare, dilute, and deliver protein and nucleic acid samples to electrophoresis gels for separation and analysis; includes solutions to stabilize, control the concentration of, denature, and reduce sample proteins.
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Keyword Search:
1 – 15 of 54 results
1
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Invitrogen™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.

EDGE
Content And Storage Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT).

Store at 2°C to 8°C.
For Use With (Equipment) Bolt Mini Gel Tank
Product Type Sample Reducing Agent
Form Liquid
Formulation 500 mM dithiothreitol (DTT)
Reagent Type Reducing Agent
Concentration 10X, 500 mM
Gel Compatibility Bolt™ Bis-Tris Plus Gels
Product Line Bolt
2
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Invitrogen™ Bolt™ Transfer Buffer (20X)

Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting

EDGE
Buffer Transfer Buffers
Content And Storage Store at 4 to 25°C.
Product Type Western Blot Transfer Buffer
Form Liquid
Concentration 20 X
Gel Compatibility Bolt™ Bis-Tris Plus Gels
Product Line Bolt
3
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Invitrogen™ TrackIt™ Cyan/Orange Loading Buffer
GREENER_CHOICE

A 6X solution used for easy loading and tracking of DNA samples in agarose gels

EDGE
4

Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311

Optimized for using with Novex™ pH 3-10 IEF gels

EDGE
5
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Invitrogen™ TrackIt™ Cyan/Yellow Loading Buffer
GREENER_CHOICE

6X solutions used for easy loading and tracking of DNA samples in agarose gels.

EDGE
6
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Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.

EDGE
Shipping Condition Room Temperature
Content And Storage Store at –20°C.
Format Bottle
Form Liquid
Gel Compatibility Denaturing Polyacrylamide Gels
Product Line Ambion
7
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Invitrogen™ NativePAGE™ 5% G-250 Sample Additive

Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels

EDGE
8

Invitrogen™ Novex™ Zymogram Developing Buffer (10X)

For use with Novex Zymogram Gels

EDGE
9
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Invitrogen™ Fluorescent Compatible Sample Buffer (4X, non-reducing)

Invitrogen Fluorescent Compatible Sample Buffer is for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE).

EDGE
10

Invitrogen™ Novex™ IEF Sample Buffer pH 3-10 (2X)

An optimized pre-mixed IEF sample buffer

EDGE
11

Invitrogen™ UltraPure™ Sodium Dodecyl Sulfate (SDS)

Detergent that functions as protein denaturant

EDGE
12
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Invitrogen™ Digitonin (5%)

Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.

EDGE
13

Invitrogen™ Novex™ IEF Cathode Buffer pH 3-7 (10X)

Optimized pre-mixed IEF buffers reduce variability and ensure consistent results

EDGE
14
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Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Buffer Sample Loading Buffers
Content And Storage Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red.

Store at 4°C to 25°C.
Gel Type NuPAGE
Product Type LDS Sample Buffer
Gel Compatibility NuPAGE™ Gels
Product Line NuPAGE
15
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Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis