PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

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Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Quantity	Each
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

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Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Pricing and Availability
Specifications

Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Spin Column
Run Time	5 min.
High-throughput Compatibility	Not High-throughput Compatible (Manual)

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Invitrogen™ Platinum™ SuperFi II DNA Polymerase

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>300X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
For Use With (Application)	Hot-start PCR,High-fidelity PCR

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Applied Biosystems™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Pricing and Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
Purity or Quality Grade	UP (Ultra Pure)
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	TaqMan
Detection Method	Primer-probe

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Invitrogen™ dNTP Set (100 mM)

Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Form	Liquid
Label or Dye	Unlabeled
Concentration	100 mM
For Use With (Application)	Standard PCR,RT-PCR,Real Time PCR (qPCR)

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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• RevertAid Reverse Transcriptase (200 U/μL) • 5X Reaction Buffer Store at –20°C.
Reverse Transcriptase	RevertAid
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

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Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

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Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	2X
Reaction Format	Separate Components
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Starting Material	DNA
For Use With (Application)	Cloning,Hot-start PCR

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Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Pricing and Availability
Specifications

Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
Concentration	5 U/μL

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

Pricing and Availability
Specifications

No. of Reactions	100 Reactions
Format	Kit
Label or Dye	FAM
Quantity	100 reactions
Host Cell	E.coli
PCR Method	qPCR
Product Line	resDNASEQ
Detection Method	Primer-probe

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Invitrogen™ dNTP Mix (10 mM ea)

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Form	Liquid
Label or Dye	Unlabeled
Concentration	10 mM
For Use With (Application)	Standard PCR,RT-PCR,Real Time PCR (qPCR)

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Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	ArrayScript™ UP
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	RNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Run Time	Standard
Product Line	RNA-to-C_T, SYBR

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Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Pricing and Availability
Specifications

Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Bottle
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type	Environmental Sample, Food
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Product Line	TaqMan

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Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
For Use With (Application)	Hot-start PCR

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Invitrogen™ Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Standard PCR
PCR Method	qPCR,Standard PCR
Exonuclease Activity	5' - 3'
Polymerase	Taq DNA Polymerase
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	No
Starting Material	DNA

PCR Equipment and Supplies

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