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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (636)
Standard PCR Products (148)
DNA Polymerases (14)
Reverse Transcription Products (3)

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1
Promotions Available

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan
2
Promotions Available

Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 10 X
Reverse Transcriptase MultiScribe™
Final Product Type cDNA
Concentration 10X
For Use With (Application) miRNA analysis
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Size (Final Product) 200 bp or less
Reaction Format Separate Components
Product Type MicroRNA Reverse Transcription Kit
Sample Type RNA
Reaction Speed Fast
Optimal Reaction Temperature 42°C
Product Line TaqMan
3
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

EDGE
GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
4
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

EDGE
5
Promotions Available

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

EDGE
Polymerase AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Multiplex Master Mix
Sample Type DNA
Reaction Speed Fast
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method Multiplex qPCR
Product Line TaqMan
Detection Method Primer-probe
6
Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
Purity or Quality Grade UP (Ultra Pure)
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
7
Promotions Available

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

EDGE
GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT, TaqMan
Detection Method TaqMan
8
Promotions Available

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

EDGE
Shipping Condition Dry Ice
Reverse Transcriptase M-MLV
Form Liquid
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Thermostability Not Applicable
Polymerase AmpliTaq Fast DNA Polymerase
Format Tube
Product Type 1-Step Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
Product Line TaqMan
9
Promotions Available

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT
10

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

EDGE
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase AmpliTaq Fast DNA Polymerase
Product Type Fast Advanced Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line TaqMan
11
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

EDGE
GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
12
Promotions Available

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
GREENER_CHOICE

The TaqMan™ Cells-to-CT™ Express Kit enables rapid, reproducible gene expression analysis directly from cultured cells—allowing you to skip RNA extraction entirely. By removing extraction steps, the workflow reduces sample loss, shortens time to results, and improves consistency across experiments and operators.

EDGE
Shipping Condition Frozen, Dry Ice
Target cDNA
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format Kit
Sample Type Mammalian Cells
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Purification Target RNA
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
13
Promotions Available

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

EDGE
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq DNA Polymerase
Format Tube
Product Type Fast Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Product Line AmpErase, TaqMan
14
Promotions Available

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR, no UNG

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time.

EDGE
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantification,Presence/Absence
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP
Thermostability 72 hr., Assembled Reaction
Polymerase AmpliTaq Fast DNA Polymerase
Product Type Fast Advanced Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line TaqMan
15
Promotions Available

Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

EDGE
Shipping Condition Wet Ice
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe