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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

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Thermo Scientific™ ABsolute™ qPCR Master Mixes, SYBR Green-Based Detection

Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.

Activation Time heated to 95°C for 15 minutes
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Verso™ 1-Step SYBR Green-Based Detection RT-qPCR Kits

Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits have been specially developed to quantify RNA in a convenient one-step assay.

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Thermo Scientific™ ABsolute Blue qPCR Mixes

Make verification of master mix dispensing quick, easy and foolproof with these colored qPCR master mixes for probe chemistry using standard cycling protocols.

Content And Storage ABsolute Blue qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated). Store at -20°C. Avoid freeze/thaw cycles. The ROX dye is light sensitive; exposure to light should be minimized for vials containing ROX dye.
Compatibility ABsolute Blue qPCR mixes are ideal for use with white reaction plates and tubes. Check qPCR cycler compatibility with our qPCR reagent selection guide.
For Use With (Application) Gene Expression
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ABsolute™ qPCR Master Mixes, SYBR Green-Based Detection

Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.