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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1 – 15 of 22 results
1
Promotions Available

Invitrogen™ Single Cell Lysis Kit
GREENER_CHOICE

Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

2

Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.

3

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
GREENER_CHOICE

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Frozen, Dry Ice
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Green Features REACH compliant, less waste
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Target cDNA
Format Kit
Sample Type Mammalian Cells
Purification Target RNA
Starting Material 10-100,000 Cells
4
Promotions Available

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
GREENER_CHOICE

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Green Features Fewer resources used and less waste, Less hazardous
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method SYBR
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT™
5
Promotions Available

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

EDGE
GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Green Features Fewer resources used and less waste, Less hazardous
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, TaqMan™
Detection Method TaqMan
6

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Green Features Fewer resources used, less waste, less hazardous
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT™
7

Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.

8

Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer

These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.

9

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Shipping Condition Dry Ice
Content And Storage 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at
-20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase M-MLV
Size (Final Product) 7 kb or less
Final Product Type cDNA
Sample Type Mammalian Cells
Purification Time 2 hr.
Optimal Reaction Temperature 42°C
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Product Line Ambion™, Cells-to-cDNA™
10

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Green Features Fewer resources used and less waste, less hazardous
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, Power SYBR™ Green
Detection Method SYBR
11
Promotions Available

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
GREENER_CHOICE

The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Shipping Condition Dry Ice
Sample Type Mammalian Cells
Reaction Speed 7 min.
Purification Time 7 min.
Green Features Fewer resources used and less waste, less hazardous
Starting Material 1-10 Cells
For Use With (Application) RT-PCR
PCR Method 2-step RT-qPCR
Product Line Ambion™, Cells-to-CT™
Detection Method TaqMan
12
Promotions Available

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
Green Features Fewer resources used and less waste, Less hazardous
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Polymerase DNA Polymerase
Format Tube
Product Type Gene Expression Cells-to-C Kit
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cell
Reaction Speed Standard
Product Line Ambion™, Cells-to-CT™, TaqMan™
13

Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit
GREENER_CHOICE

Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Shipping Condition Dry Ice
Content And Storage Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature.
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Green Features Fewer resources used and less waste, Less hazardous
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Fast DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type Cells
Reaction Speed Fast
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT™, SYBR™
14

Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
GREENER_CHOICE

Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Shipping Condition Dry Ice
Purity or Quality Grade LD (Low DNA)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Green Features Fewer resources used and less waste, Less hazardous
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Standard
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT™, SYBR™
15
Promotions Available

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit
GREENER_CHOICE

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.