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Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
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1
Promotions Available

Applied Biosystems™ Absolute Q™ MAP16 Plate Kit and Absolute Q™ Universal DNA dPCR Master Mix

Eliminate the need to design custom assays and choose from over 20 million predesigned assays. Includes the Absolute Q MAP16 plate kit plus the Absolute Q Universal DNA dPCR master mix which is compatible with a wide range of applications and difficult targets.

2
Promotions Available

Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)

Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.

3
Promotions Available

Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)

Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.

4
Promotions Available

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

5
Promotions Available

Applied Biosystems™ Absolute Q™ MAP16 Plate Kit and 1-Step RT-dPCR Master Mix

A key component of the QuantStudio Absolute Q Digital PCR System which is powered by the patented microfluidic array plate (MAP) technology that enables consistent and automated sample separation.

6
Promotions Available

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, low ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute Blue
Detection Method SYBR
7
Promotions Available

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility Block-Based qPCR Instruments
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute Blue
Detection Method SYBR
8

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute
Detection Method SYBR
9
Promotions Available

Thermo Scientific™ ABsolute Blue QPCR Mix, ROX

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Format Tube
Product Type qPCR Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
PCR Method qPCR
Product Line ABsolute Blue
Detection Method Primer-probe
10
Promotions Available

Thermo Scientific™ ABsolute QPCR Mix, ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method Primer-probe
11
Promotions Available

Thermo Scientific™ ABsolute QPCR Mix, no ROX

Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Polymerase Taq DNA Polymerase
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
PCR Method qPCR
Product Line ABsolute
Detection Method Primer-probe
12
Promotions Available

Thermo Scientific™ ABsolute QPCR Mix, low ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method Primer-probe
13
Promotions Available

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, low ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method SYBR
14

Thermo Scientific™ ABsolute Blue QPCR Mix, low ROX

ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Format Tube
Product Type qPCR Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute Blue
Detection Method Primer-probe
15
Promotions Available

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, no ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method SYBR