The streptavidin, horseradish peroxidase (HRP) conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic HRP substrates.
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NeutrAvidin Protein, HRP conjugate, 31001, from Invitrogen™, 31001, from Invitrogen™ Species Reactivity: ; Applications: ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot.
NeutrAvidin Protein, AP conjugate, 31002, from Invitrogen™, 31002, from Invitrogen™ Species Reactivity: ; Applications: ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot.
Perform Western blot, ELISA or other biotin-based detection with purified and HRP-, AP-, FITC-, TRITC-, PE- or APC-conjugated or maleimide-/hydrazide-activated streptavidin.
Perform Western blot, ELISA or other biotin-based detection with purified and HRP-, AP-, FITC-, TRITC-, PE- or APC-conjugated or maleimide-/hydrazide-activated streptavidin.
LanthaScreen™ Tb-Streptavidin is supplied at 1 mg/ml and contains the streptavidin in HEPES buffered saline, 400 μl of the fluorescein-labeled, biotinylated peptide for use as a positive control, and 100 ml of TR-FRET Dilution Buffer. Store components as indicated in the product Certificate of Analysis (-20°C, or +20-30°C).
R&D Systems™ Strep-Diluent is an optimized blocking reagent specifically developed to dilute Strep-HRP and block unspecific binding in the TACS XL™ line of kits.
For use in ELISAs, dilute to the working concentration specified on the vial label using 1% BSA/PBS or Reagent Diluent Concentrate 2 (Catalog # DY995) diluted 1:10 in deionized water.