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Thermo Scientific™ ELISA Carbonate Coating Buffer
For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | EDC |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| PEGylated | No |
Invitrogen™ ELISA TMB Stabilized Chromogen
For use with all sandwich ELISAs which utilize Horseradish Peroxidase (HRP) as detecting enzyme
Thermo Scientific™ Mass Spectrometry Grade Proteases
Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.
| Workflow Step | Protein Digestion |
|---|---|
| For Use With (Equipment) | Mass Spectrometer |
| Grade | MS |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ Micro BCA™ Reagent A (MA)
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.
Thermo Scientific™ Micro BCA™ Reagent B (MB)
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Gibco™ ExpiSf™ Protein Production Kit
Gibco™ ExpiSf™ Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells using the ExpiSf Expression System.
| Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
|---|---|
| Expression Mechanism | Cell-Based Expression |
| Format | Liquid |
| Compatible Cells | Insect Cells |
| Classification | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
| Expression System | Baculovirus |
| Product Line | ExpiSf |
Molecular Probes™ Alexa Fluor™ 488 Azide (Alexa Fluor™ 488 5-Carboxamido-(6-Azidohexanyl), Bis(Triethylammonium Salt)), 5-isomer
The green-fluorescent Alexa Fluor™ 488 azide is reactive with terminal alkynes via a copper-catalyzed click reaction.
Invitrogen™ Alexa Fluor™ 568 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 568 is a bright orange dye. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 568 dye is water soluble and pH-insensitive from pH 4 to pH 10.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 568 |
| Reactive Moiety | Active Ester, Succinimidyl Ester |
| Emission | 602 nm |
| Excitation | 578 nm |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor |
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
| Content And Storage | Store desiccated at -20°C, protect from moisture |
|---|---|
| Product Type | NHS-Fluorescein |
| Label or Dye | FITC (Fluorescein) |
| Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
| Solubility | DMF (Dimethylformamide) |
| Emission | 518 |
| Excitation | 494 |
| Label Type | Classic Dyes |
| Chemical Reactivity | Amine |
| Product Line | Pierce |
Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate
HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.
| Content And Storage | Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction |
|---|---|
| Substrate Properties | Chemical Substrate |
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Product Line | SuperSignal |
| Detection Method | Chemiluminescence |
Thermo Scientific™ Pierce™ Dye Removal Columns
Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.
Thermo Scientific™ Pierce™ 660nm Protein Assay Kit
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Invitrogen™ Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Fluorescein-5-Isothiocyanate |
| Label or Dye | FITC (Fluorescein) |
| Reactive Moiety | Isothiocyanate |
| Label Type | Classic Dyes |
| Chemical Reactivity | Amine |