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Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells
Capable of supporting site-specific recombination between pFastBac™ and bMON14272
Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Unstable DNA | No |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Preparing Unmethylated DNA | No |
| Plasmid | May be used for plasmids >20 kb |
| Reduces Recombination | Yes (recA13) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Format | Tube |
| Product Type | Electrocompetent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot™ |
| Contains F' Episome | No |
Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells
MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.
Invitrogen™ ElectroMAX™ Stbl4™ Competent Cells
ElectroMAX Stbl4 Competent Cells are specifically designed for cloning unstable inserts. This strain is a derivative of Stbl2 cells (engineered from JM109/J5 strain).
Invitrogen™ MAX Efficiency™ DH10B Competent Cells
MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.
Invitrogen™ One Shot™ BL21-AI™ Chemically Competent E. coli
One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems.
Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells
MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.
Invitrogen™ ElectroMAX™ DH5α-E Competent Cells
ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation.
Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.
Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells
One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain and carry the tonA (fhuA) mutation that confers resistance to T1 and T5 phage.
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
| Format | Kit |
|---|---|
| Product Type | Cloning Kit |
| Cloning Method | TOPO™-TA |
| Bacterial or Yeast Strain | TOP10 |
| Vector | TOPO-TA Cloning Vectors |
| Product Line | One Shot™ |
Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System
Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.
| Content And Storage | This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase. Store the product at 4°C. |
|---|---|
| Form | Liquid |
| Readout | End Point |
| Compatible Cells | Mammalian Cells |
| Target Enzyme | Alkaline Phosphatase |
| Substrate Type | AP (Alkaline Phosphatase) Substrate |
| For Use With (Application) | Enzyme Assay |
| High-throughput Compatibility | High-throughput Compatible |
| Detection Method | Chemiluminescence |
| Substrate | CSPD™ |
| Assay | Reporter Assay |
| Substrate Properties | Chemical Substrate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Product Type | Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System |
| Sample Type | Cell Cultures |
| Label or Dye | Unconjugated |
| Product Line | NovaBright™, Phospha-Light™ |
Thermo Scientific™ DH10B Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.