Biological Buffers

MES, 0.5M buffer soln., pH 9.0, Alfa Aesar™

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00006181 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O 100ML MES, 0.5M buffer soln., pH 9.0 100ml

HEPES sodium salt, >98%, MP Biomedicals™

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.284 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+] 100GR HEPES

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled TRISGLY TRANS BUF (25X)500MLNovex® Tris-Glycine Transfer Buffer (25X) is

Invitrogen™ SDS, 10% Solution

Ready-to-use, certified RNase-free 500 ML SDS, 10% SOLUTION 500ML STORE AT ROOMtemperature

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

500 ML NUCLEASE-FREE WATER (NOT DEPC-TREATED) (1x 500mL) 500mL Store at room temperature

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 1KG HEPES (Fine White Crystals) for Molecular Biology,

BICINE, 99%, Alfa Aesar™

CAS: 150-25-4 Molecular Formula: C6H13NO4 Molecular Weight (g/mol): 163.173 MDL Number: MFCD00004295 InChI Key: FSVCELGFZIQNCK-UHFFFAOYSA-N PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: C(CO)N(CCO)CC(=O)O BICINE, 99% 250G

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Thermo Scientific™ Tricine, ≥98.5% (Dry Basis), Ultrapure, Thermo Scientific™

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-Tris(Hydroxymethyl)methylglycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO 100G Tricine, 98.5+% (dry basis), Ultrapure, Thermo Scientific

Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions. 1 LT (A) IGG BINDING BUFFER, IMMUNOPURE 1L

Alfa Aesar™ ACES, 0.5M buffer soln., pH 6.5

CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N 250ML ACES, 0.5M buffer soln., pH 6.5 250ml

Alfa Aesar™ PIPES, 1.0M buffer soln., pH 8.0

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O 500ML PIPES, 1.0M buffer soln., pH 8.0 500ml

Invitrogen™ Tris (1M), pH 7.0

Ready-to-use, certified RNase-free 1 M TRIS, PH 7.0 100MLAmbion®  Molecular biology grade, 1 M Tris, pH

Alfa Aesar™ PIPES, 1.0M buffer soln., pH 7.5

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O 500ML PIPES, 1.0M buffer soln., pH 7.5 500ml

Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats X10 BOLT SAMPLE RED AGT

Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit

Optimized for using with Novex™ pH 3-10 IEF gels IEF BUFFER KIT PH 3 TO 10 00

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility. 1 LT BLOCKING BUFFER IN TBS, STARTINGBLOCK 1LStore in Fridge at 4C

Invitrogen™ DEPC-Treated Water

100 ML DEPC-TREATED WATER (1 X 100ML) 100ML STOREat room temperature

Thermo Scientific™ Pierce™ Poly HRP Dilution Buffer

Specifically designed to optimize performance of Poly-HRP Streptavidin 1 LT POLY-HRP DILUTION BUFFER

Invitrogen™ UltraPure™ Molecular Probes™ Guanidine Isothiocyanate

Strong denaturing agent GUANIDINE ISOTHIOCYANATE

Thermo Scientific™ HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure, Thermo Scientific™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 100ML HEPES, 1M soln, pH 7.3

PIPES, disodium salt, 99%, ACROS Organics™

CAS: 76836-02-7 Molecular Formula: C8H16N2Na2O6S2 Molecular Weight (g/mol): 346.324 InChI Key: GMHSTJRPSVFLMT-UHFFFAOYSA-L Synonym: 1, 4-Piperazinediethanesulfonic acid disodium salt PubChem CID: 173553 ChEBI: CHEBI:63055 IUPAC Name: disodium;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+] 25GR PIPES, disodium salt, 99%

Invitrogen™ TE, pH 7.0

Nuclease-free, ultrapure solution for RNA samples 50 ML TE, PH 7.0 50ML STORE AT ROOM TEMPERATURE

Invitrogen™ Acrylamide/Bis 19:1, 40% (w/v) solution

Ready-to-use solution reduces acrylamide and bis-acrylamide powder contact hazards 500 ML ACRYLAMIDE BIS 19:1 40% SOLUTION 500MLStore at 4°C

Alfa Aesar™ POPSO, 0.2M buffer soln., pH 8.0

CAS: 108321-07-9 Molecular Formula: C10H20N2Na2O8S2 Molecular Weight (g/mol): 406.376 MDL Number: MFCD00070005 InChI Key: XMRCLLXGMGEVHS-UHFFFAOYSA-L PubChem CID: 53393457 IUPAC Name: disodium;2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazin-1-yl]propane-1-sulfonate SMILES: C1CN(CCN1CC(CS(=O)(=O)[O-])O)CC(CS(=O)(=O)[O-])O.[Na+].[Na+] 100ML POPSO, 0.2M buffer soln., pH 8.0 100ml

Bicine, Fisher BioReagents

CAS: 150-25-4 Molecular Formula: C6H13NO4 Molecular Weight (g/mol): 163.173 InChI Key: FSVCELGFZIQNCK-UHFFFAOYSA-N Synonym: N, N-bis [2-hydroxyethyl]glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: C(CO)N(CCO)CC(=O)O 100GR Bicine (White Powder)

Alfa Aesar™ TES, 0.2M buffer soln., pH 7.5

CAS: 70331-82-7 Molecular Formula: C6H14NNaO6S Molecular Weight (g/mol): 251.229 MDL Number: MFCD00065482 InChI Key: GUXMFAHOYNRHBI-UHFFFAOYSA-M PubChem CID: 23702152 IUPAC Name: sodium;2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonate SMILES: C(CS(=O)(=O)[O-])NC(CO)(CO)CO.[Na+] 100ML TES, 0.2M buffer soln., pH 7.5 100ml

Alfa Aesar™ MES, 0.5M buffer soln., pH 7.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O 250ML MES, 0.5M buffer soln., pH 7.0 250ml

Alfa Aesar™ PIPES, 0.5M buffer soln., pH 5.5

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O 250ML PIPES, 0.5M buffer soln., pH 5.5 250ml

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