Enzymes and Inhibitors

Thermo Scientific™ HindIII

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. 25000 UNIT RESTRICTION ENZYME, CONVENTIONAL,HindIII, 50u/uL,HC, cuts A¬AGCTT at 37°C (supplied

Thermo Scientific™ FastDigest Eco91I

Cut at G^GTNACC sites with Thermo Scientific™ FastDigest™ Eco91I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST ECO91I 200UL

Thermo Scientific™ FastDigest DrdI (AasI)

Cut at GACNNNN^NNGTC sites with Thermo Scientific™ FastDigest™ DrdI (AasI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest AasI, 25µL

Thermo Scientific™ FastDigest SfiI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 150 UL RESTRICTION ENZYME, FASTDIGEST, SFII, CUTSGGCCNNNN¬NGGCC, 1µL cleaves 1µg DNA in 15 minutes

Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 50U/UL 25000U

Thermo Scientific™ FastDigest HpaI (KspAI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest KspAI, 50µL

Thermo Scientific™ SfiI

Cut at GGCCNNNN^NGGCC sites with SfiI restriction enzyme, which performs best at 50°C in G buffer. SFII 10U/UL 1000U

Thermo Scientific™ FastDigest MluI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST MLUI 100UL

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5 to 15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI. FASTDIGEST NHEI 50UL

Thermo Scientific™ FastDigest™ XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 750 UL RESTRICTION ENZYME, FASTDIGEST, XBAI, CUTST¬CTAGA, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ FastDigest NheI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 100 UL RESTRICTION ENZYME, FASTDIGEST, NHEI, CUTSG¬CTAGC, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ BcuI (SpeI)

Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI). RESTRICTION ENZYME, CONVENTIONAL, BCUI (SPEI),10u/ul, cuts A¬CTAGT at 37°C (supplied with 1mL

Thermo Scientific™ 10x Buffer Tango

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability. X5 10X Buffer Tango Unit Size 5x1.0 mL

Thermo Scientific™ XbaI

Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer. XBAI 10U/UL 3000U

Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA). TBE BUFFER 1L 10X

Thermo Scientific™ NdeI

The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI). 2500 UNIT RESTRICTION ENZYME, CONVENTIONAL, NDEI,10u/uL, cuts CA¬TATG at 37°C (suppliedwith 1mL of

Invitrogen™ Anza™ 2 NcoI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. Anza 2 NcoI (4000 units)

Invitrogen™ Anza™ 27 PvuI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. 400 Units Anza 27 PvuI

Thermo Scientific™ PvuI

The PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer (Isoschizomers: BpvUI, MvrI, Ple19I). RESTRICTION ENZYME, CONVENTIONAL, PVUI, 10U/UL,cuts CGAT¬CG at 37°C (suppliedwith 1mL of 10X

Thermo Scientific™ MvaI (BstNI)

Cut at CC^WGG sites with MvaI (BstNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI). 2000 UNIT RESTRICTION ENZYME, CONVENTIONAL, MVAI(BstNI), 10u/uL, cuts CC¬WGG at 37°C (supplied

Thermo Scientific™ FastDigest MauBI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 20 UL RESTRICTION ENZYME, FASTDIGEST, MAUBI, CUTSCG¬CGCGCG, 1µL cleaves 1µg DNAin 5 minutes at

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI). DPNI 10U/UL 2500U

Thermo Scientific™ MreI (Sse232I)

Cut at CG^CCGGCG sites with MreI (Sse232I) restriction enzyme, which performs best at 37°C in G buffer. MREI (SSE232I) 10U/UL 300U

Thermo Scientific™ FastDigest™ SpeI (BcuI)

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI). Restriction enzyme, FastDigest BcuI, 20µL

Thermo Scientific™ Eco57I (AcuI)

The Eco57I (AcuI) restriction enzyme recognizes CTGAAG(16/14)^ sites and cuts best at 37°C in G (+SAM) buffer (Isoschizomers: AcuI). ECO57I (ACUI) 5U/UL 200U

Thermo Scientific™ FastDigest TatI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 20 UL RESTRICTION ENZYME, FASTDIGEST, TATI, CUTSW¬GTACW, 1µL cleaves 1µg DNA in 15 minutes at

OPTIZYME™ RsaI, Fisher BioReagents™

Optizyme RSA I restriction enzyme

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 20 UL RESTRICTION ENZYME, FASTDIGEST, NOTI, CUTSGC¬GGCCGC, 1µL cleaves 1µg DNA in 5 minutes at

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