Enzymes and Inhibitors

Thermo Scientific™ HpaII

The HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. HPAII 10U/UL 5000U

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ BglII

Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer. BGLII 10U/UL 2500U

Thermo Scientific™ FastDigest HpaI (KspAI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest KspAI, 50µL

Thermo Scientific™ FastDigest MssI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST MSSI 100UL

Thermo Scientific™ FastDigest AflII (BspTI)

Cut at C^TTAAG sites with Thermo Scientific™ FastDigest™ AflII (BspTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest BspTI, 50µL

Thermo Scientific™ FastDigest RsrII (CpoI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest CpoI, 20µL

Thermo Scientific™ FastDigest SbfI (SdaI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest SdaI, 20µL

Thermo Scientific™ SgrDI

Cut at CG^TCGACG sites with SgrDI restriction enzyme, which functions best at 37°C in R buffer. RESTRICTION ENZYME, CONVENTIONAL, SGRDI, 5U/UL,cuts CG¬TCGACG at 37°C (supplied with 1mL of 10X

Thermo Scientific™ FastDigest MreI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 20 UL RESTRICTION ENZYME, FASTDIGEST, MREI, CUTSCG¬CCGGCG, 1µL cleaves 1µg DNAin 5 minutes at

OPTIZYME™ Sau3AI (Bsp143I), Fisher BioReagents™

Optizyme Sau3A I restriction enzyme

Thermo Scientific™ FastDigest BstZ17I (Bst1107I)

Cut at GTA^TAC sites with Thermo Scientific™ FastDigest™ BstZ17I (Bst1107I), which performs best using universal FastDigest Buffer (Isoschizomers: BssNAI). Restriction enzyme, FastDigest Bst1107I, 100µL

Thermo Scientific™ FastDigest™ ClaI (Bsu15I)

Cut at AT^CGAT sites with ClaI (Bsu15I) restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer. Restriction enzyme, FastDigest Bsu15I, 100µL

Thermo Scientific™ FastDigest PacI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 25 UL RESTRICTION ENZYMES, ,FastDigest PacI, 25µL, cuts TTAAT¬TAA, 1µL cleaves

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5 to 15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI. FASTDIGEST NHEI 50UL

Thermo Scientific™ FastDigest BclI

Cut at T^GATCA sites with Thermo Scientific™ FastDigest™ BclI, which performs best at 55°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FbaI, Ksp22I). FASTDIGEST BCLI 300UL

Thermo Scientific™ FastDigest Sau3AI (Bsp143I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Bsp143I, 40µL

Thermo Scientific™ HindIII

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. 25000 UNIT RESTRICTION ENZYME, CONVENTIONAL,HindIII, 50u/uL,HC, cuts A¬AGCTT at 37°C (supplied

Thermo Scientific™ Tru1I (MseI)

Cut at T^TAA sites with Tru1I (MseI) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: MseI, Tru9I). 1500 UNIT RESTRICTION ENZYME, CONVENTIONAL, TRU1I(MseI), 10u/ul, cuts T¬TAA at 65°C (supplied with

Thermo Scientific™ FastDigest SapI (LguI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest LguI, 20µL

Thermo Scientific™ DreamTaq Buffers

Optimize PCR with Thermo Scientific™ DreamTaq DNA Polymerase with these buffers. X4 10X DREAMTAQ BUFFER 1.25ML

Thermo Scientific™ XbaI

Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer. XBAI 10U/UL 5X1500U

Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I). 300 UL RESTRICTION ENZYME, FASTDIGEST, NCOI, CUTSC¬CATGG, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ Eco32I (EcoRV)

The Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV). ECO32I (ECORV) 10U/UL 4000U

Thermo Scientific™ FastDigest™ DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI). 100 UL RESTRICTION ENZYME, FASTDIGEST, DPNI, CUTSGA¬TC, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 10U/UL 10000U

Thermo Scientific™ Eco88I (AvaI)

Cut at C^YCGRG sites with Eco88I (AvaI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBIm). ECO88I (AVAI) 10U/UL 1000U

Thermo Scientific™ FastDigest AvrII (XmaJI)

Cut at C^CTAGG sites with Thermo Scientific™ FastDigest™ AvrII (XmaJI), which performs best using universal FastDigest Buffer (Isoschizomers: AspA2I, BlnI). Restriction enzyme, FastDigest XmaJI, 20µL

Thermo Scientific™ FastDigest SanDI (KflI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest KflI, 20µL

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