Chromatography Media
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Filtered Search Results
Cytiva Butyl-S Sepharose™ 6 Fast Flow Chromatography Media
The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: DEAE
Used for preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ Sephaorose™ Fast Flow Anion Exchange Media: DEAE ensures low levels of non-specific binding.
Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin
Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | Strep-Tactin XT |
| Density (Metric) | Approx. 5 mg/mL resin |
| Matrix | Rigid, cross-linked agarose, spherical |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 6 to 10 pH |
| Binding Capacity | Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin |
| Storage Requirements | 2 to 8°C in 20% ethanol |
| For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag |
Applied Biosystems™ Trifluoroacetic Acid (TFA) Neat Liquid
Trifluoroacetic Acid (TFA, concentrated) for use with OPC™ cartridges for oligonucleotide purification.
Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Flow Rate | 1 to 4 mL/min |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | HiTrap Talon crude prepacked Column |
| Material | Polypropylene |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
| Max. Pressure | 3 bar |
Cytiva DEAE Sepharose™ Fast Flow Chromatography Media
For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.
Cytiva SP Sepharose™ High Performance Media
Use for high intermediate and polishing steps in downstream processing. Cytiva Life Sciences™ SP Sepharose™ High Performance Media is composed of crosslinked agarose beads.
Cytiva His SpinTrap™ TALON™
Single-use spin column for high purity purification of histidine-tagged proteins
Cytiva CNBr-Activated Sepharose™ 4 Fast Flow Affinity Media
Used for coupling of large amino-containing ligands
Cytiva rProtein A Sepharose™ Fast Flow Affinity Media
Designed for purification of monoclonal and polyclonal antibodies at labs and process scales
| Particle Size | 60 to 165 μm |
|---|---|
| Ligand Type | Recombinant Protein A (E. coli) |
| Flow Rate | 150 to 250 cm/Hoμr |
| Matrix | 4% Cross-Linked Agarose |
| Stability | Stable in aqueous Buffers commonly used in Protein A Chromatography |
| Storage Buffer | 20% Ethanol |
| pH Range | 2 to 9 |
| Product Type | rProtein A Sepharose Fast Flow Media |
| Height (English) Bed | 9.84 in. |
| Storage Requirements | 2°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales |
| Height (Metric) Bed | 25 cm |
| Max. Pressure | 100 kPa |
Cytiva Sepharose™ Fast Flow Cation Exchange Media
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
| Particle Size | 45 to 165 μm |
|---|---|
| pH Range | 4 to 13 |
Cytiva TALON™ Superflow Medium
Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | Talon Superflow Media |
| Binding Capacity | Up to 20 mg Histidine-tagged Protein |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography |
Cytiva Capto™ Q Chromatography Media
Designed for capture and intermediate purification of proteins from large feed volumes. Cytiva CaptoTM Q Chromatography Media is a strong anion exchange BioProcess medium, designed to meet the demands of modern large-scale manufacturers.
Cytiva Phenyl Sepharose™ High Performance Media
Designed for intermediate and polishing step purification steps