Chromatography Media

Resins or particles used to separate components of a sample via column chromatography; products include media used in cation or anion exchange, fast flow, and high-performance chromatography techniques, etc.

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Cytiva TALON™ Superflow Medium

Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.

Pricing and Availability
Specifications

Particle Size	60 to 160 μm
Ligand Type	Talon Superflow Cobalt-IMAC
Matrix	6% Cross-Linked Agarose
Storage Buffer	20% Ethanol
pH Range	3 to 12
Product Type	Talon Superflow Media
Binding Capacity	Up to 20 mg Histidine-tagged Protein
Storage Requirements	4°C to 8°C
For Use With (Application)	For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography

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Cytiva Butyl-S Sepharose™ 6 Fast Flow Chromatography Media

The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process

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Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin

Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.

Pricing and Availability
Specifications

Particle Size	34 μm
Ligand Type	Strep-Tactin XT
Density (Metric)	Approx. 5 mg/mL resin
Matrix	Rigid, cross-linked agarose, spherical
Stability	Stable in all commonly used aqueous buffers
pH Range	6 to 10 pH
Binding Capacity	Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin
Storage Requirements	2 to 8°C in 20% ethanol
For Use With (Application)	Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Applied Biosystems™ Trifluoroacetic Acid (TFA) Neat Liquid

Trifluoroacetic Acid (TFA, concentrated) for use with OPC™ cartridges for oligonucleotide purification.

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Cytiva HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Pricing and Availability
Specifications

Particle Size	60 to 160 μm
Ligand Type	Talon Superflow Cobalt-IMAC
Flow Rate	1 to 4 mL/min
Matrix	6% Cross-Linked Agarose
Storage Buffer	20% Ethanol
pH Range	3 to 12
Product Type	HiTrap Talon crude prepacked Column
Material	Polypropylene
Storage Requirements	4°C to 8°C
For Use With (Application)	For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Max. Pressure	3 bar

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Cytiva Q Sepharose™ High Performance Chromatography Media

Designed for high intermediate and polishing steps in downstream processing

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Cytiva MabSelect Sure™ LX Chromatography Media

Protein A affinity medium with high dynamic binding capacity which improves performance and cuts costs

Pricing and Availability
Specifications

Stability	Stable in aqueous Buffers commonly used in Protein A Chromatography
Storage Buffer	20% Ethanol
pH Range	3 to 12
Height (English) Bed	7.87 in.
Storage Requirements	2°C to 8°C
For Use With (Application)	For PurifiCatIon of Monoclonal Antibodies for Clinical AppliCatIons
Max. Pressure	<2 bar
Includes	Certificate of Analysis
Particle Size	85 μm
Ligand Type	Alkali-stabilized Protein A-derived (E. coli)
Flow Rate	500 cm/Hoμr
Matrix	Highly Cross-Linked Agarose
Product Type	MabSelect SuRe LX Media
Binding Capacity	60 mg Human IgG/mL mediμm
Height (Metric) Bed	20 cm

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Cytiva SP Sepharose™ High Performance Media

Use for high intermediate and polishing steps in downstream processing. Cytiva Life Sciences™ SP Sepharose™ High Performance Media is composed of crosslinked agarose beads.

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Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Pricing and Availability
Specifications

Particle Size	60 to 165 μm
Ligand Type	Recombinant Protein A (E. coli)
Flow Rate	150 to 250 cm/Hoμr
Matrix	4% Cross-Linked Agarose
Stability	Stable in aqueous Buffers commonly used in Protein A Chromatography
Storage Buffer	20% Ethanol
pH Range	2 to 9
Product Type	rProtein A Sepharose Fast Flow Media
Height (English) Bed	9.84 in.
Storage Requirements	2°C to 8°C
For Use With (Application)	For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
Height (Metric) Bed	25 cm
Max. Pressure	100 kPa

Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media

Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases

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Cytiva Capto™ MMC ImpRes Multimodal BioProcess™ Chromatography Medium

For high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules

Pricing and Availability
Specifications

Particle Size	40 μm
Ligand Type	Multimodal Weak CatIon Exchanger
Matrix	High-flow Agarose
Stability	1 M Acetic Acid, 1 M NaOH, 2 M NaCl, 5% 1-Propanol, 30% Isopropanol, 70% Ethanol
Storage Buffer	20% Ethanol, 0.2 M Sodium Acetate
pH Range	3 to 12
Product Type	Capto MMC ImpRes Media
Binding Capacity	60 to 90 mg Mab/mL
Anion Exchange Capacity	25 to 39 μM/mL
Storage Requirements	4°C to 30°C
For Use With (Application)	For High Resolution Polishing of Monoclonal Antibodies (MAbs) and Other Biomolecules
Max. Pressure	300 kPa

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Cytiva DEAE Sepharose™ Fast Flow Chromatography Media

For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.