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Abnova™ 293T Whole Cell Lysate (Denatured)

Product Code. 16089645
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. Quantity unitSize
16089645 100 μL 100µL
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Product Code. 16089645 Supplier Abnova™ Supplier No. L151V1.100uL

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Description

Human Cell Line Lysate

Specifications

Cell Line 293T
For Use With (Application) Western Blot
Tissue Kidney
Description Whole cell lysate (denatured)
Lysis Buffer 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Preparation Method Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 100 μL
Storage Buffer In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Host Species Human
Content And Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Safety and Handling

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Product Identifier
  • 100UL 293T whole cell lysate (denatured)
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P102-Keep out of reach of children.
  • P233-Keep container tightly closed.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : 2-mercaptoethanol
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