Molecular Probes™ AbC™ Total Antibody Compensation Bead Kit
AbC™ Total Antibody Compensation Bead Kit
Brand: Molecular Probes™ A10497
Additional Details : Poids : 0.02000kg
DescriptionThe AbC™ Total Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry.
Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Total Antibody Compensation Bead Kit was developed to address this issue.
How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse, rat, hamster, or rabbit antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.
Broadest multispecies reactivity
The AbC™ Total Antibody Compensation Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM), rat (IgG1, IgG2a, IgG2b, IgG2c, IgM), hamster (Armenian IgG and Syrian IgG), and rabbit (monoclonal and polyclonal) immunoglobulins.
Brightest signalThe AbC™ Total Compensation Bead Kit demonstrates the highest and most consistent reactivity to the different subclasses of immunoglobulin in the industry.
Maximum laser compatibility
All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.
|Flow Cytometry Compensation Beads|
|any lasers (UV to 633/635)|
|Contains 1 vial AbC™ Total Compensation capture beads and 1 vial of negative beads.
Store at 2-8°C.
For Research Use Only. Not for use in diagnostic procedures.