missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Lysates
  5. Cell Line Lysates
  6. 16079615

Abnova bmMSCs(Human bone marrow-derived mesenchymal stem cell) whole cell lysate (nondenatured)

Product Code. 16079615
Change view
Click to view available options
Quantity:
50 μg
Unit Size:
50µg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
16079615 50 μg 50µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 16079615 Supplier Abnova Supplier No. L047V2.50ug

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

  • Stem cells maintained in the undifferentiated state using specialized culturing protocols
  • Monitored for undifferentiated cell morphology
  • Analyzed using Flow Cytometry against specific cell marker
  • Coommasie Blue Staining of total cell lysate in SDS-PAGE

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application) Immunoprecipitation, Western Blot
Tissue Bone Marrow
Description Whole cell lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 50 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Human
Concentration 4 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.