alpha-Fetoprotein, Alexa Fluor 488, clone: AFP3, eBioscience™
Mouse Monoclonal Antibody
Marque: Affymetrix eBioscience 53-6583-80
Informations supplémentaires : Poids : 0.25000kg
DescriptionDescription: This AFP3 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions.When performing western blotting or immunohistochemistry on paraffin section, we recommend the use of monoclonal antibody 1E8 (cat. 14-9499).Applications Reported: This AFP3 antibody has been reported for use in immunocytochemistry.Applications Tested: This AFP3 antibody has been tested by immunofluorescent staining of formaldehyde-fixed and permeabilized HepG2 cells. This can be used at less than or equal to 10μg/ml. It is recommended that the antibody be titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|alpha-fetoglobulin, alpha-1-fetoprotein, AFP|
|Alexa Fluor 488|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.