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Invitrogen™ Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG
Features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody. Alexa Fluor™ 488 Tyramide SuperBoost™ Kit combine brightness of AlexaFluor dyes with high signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce s
Brand: Invitrogen™ B40943
Additional Details : Weight : 0.15430kg
Description
SuperBoost™ tyramide signal amplification is most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).Features:
- High sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
- Simple protocol and detection using standard filters
- Suitable for high-resolution multiplex images, co-label with DAPI, secondary antibodies, and other SuperBoost kits
- Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
Benefits:
- Enhancement of signal using Alexa Fluor tyramides: Utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules
- Poly-HRP enhancement: Several HRP enzymes are conjugated with short polymers, enhancing signal by several fold over regular HRP systems
- Reaction stop solution: Include an HRP stop solution to halt the HRP reaction
- Reduction of background: Include blockers for elimination or reduction of endogenous peroxidase and fluorescent background signals
Shelf Life:
- Stable for 6 months after receiving
Specifications
| Alexa Fluor 488 | |
| 1 kit sufficient for 50 microscope slides (18 x 18 mm), containing: • Blocking buffer (1X), 22.5 mL • Poly-HRP-conjugated goat anti-rabbit secondary antibody (1X), 22.5 mL • Alexa Fluor tyramide reagent • Hydrogen peroxide (stabilized 3% solution), 28.5 mL • Reaction buffer (20X), 6 mL • Reaction stop solution, 8 mg • Dimethylsulfoxide (DMSO), 200 μL Store at 2-8°C. When stored as directed, the product is stable for 6 months after receiving. |
|
| Approved for shipment at room temperature or on wet ice | |
| 50 Slides |
For Research Use Only. Not for use in diagnostic procedures.