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Invitrogen™ NorthernMax™-Gly Kit
An alternative to formaldehyde-based systems for northern analysis and uses a glyoxal/DMSO Loading Solution for sample denaturation
Brand: Invitrogen™ AM1946
Additional Details : Weight : 4.28000kg
Includes:
Positive Control RNA, pTRI-GAPDH Mouse, DECAtemplate §-actin-Mouse, Formaldehyde Load Dye, ULTRAhyb Buffer, 10X Denaturing Gel Buffer, and 10X MOPS Gel Running Buffer, Agarose-LE, Transfer Buffer, Low Stringency Wash Solution No. 1, High Stringency Wash Solution No. 2, and RnaseZap™, nuclease-free water
Description
- Use of glyoxal/DMSO instead of formaldehyde for sample preparation avoids the need to pour and run gels in a fume hood, and eliminates the safety issues associated with use of formaldehyde
- Glyoxal gels can be prepared at the lab bench: ;no noxious reagents
- Increased sensitivity up to 100x over standard hybridization protocols
- Reduce hybridization to 2 hours for many messages
- Complete kit contains ULTRAhyb™ Hybridization Buffer and gel and wash reagents for 10 to 20 gels
- Compatible with RNA, DNA, or oligonucleotide probes labeled isotopically or nonisotopically
- Sufficient reagents are provided for processing 1000cm2 membrane
- RNA samples denatured with glyoxal may show sharper bands on northern blots compared to samples denatured and run in the presence of formaldehyde
- The streamlined NorthernMax-Gly procedure is more convenient and faster than the standard glyoxal gel protocol: A single reagent is used for sample denaturation and loading, the incubation time for sample denaturation has been reduced, and no recirculation of buffer is required during electrophoresis
- The volume ratio of glyoxal denaturation solution to sample RNA is lower than many protocols, so that sample precipitation prior to gel loading is usually not required
- An RNA sample and template for probe synthesis are also provided as controls for each kit
- Compatible with glyoxal-containing gels
DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting
Order Info
Shipping Condition: Room Temperature
Specifications
| • Positive Control RNA, pTRI-GAPDH Mouse, DECAtemplate §-actin-Mouse, and Glyoxal Load Dye should be stored at -20°C. • ULTRAhyb™ Buffer and 10X Gel Prep⁄Running Buffer should be stored at 4°C. • Agarose-LE™, Transfer Buffer, Low Stringency Wash Solution #1, mL High Stringency Wash Solution #2, and RNaseZap™ should be stored at room temperature. • Nuclease-free water may be stored at any temperature. |
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| Northern Analysis Reagents | |
| Glyoxal-Containing Gels | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.