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Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit

Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

Brand:  Invitrogen™ 12173011A

1142.72 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.56000kg


Product Code. 12055825

  • 1714.00€ / Pack of 4
Estimated Shipment: 12-04-2024
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Description

Description

Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Specifications

Specifications

• 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature
• 250 mL PureLink Pro 96 Wash Buffer I; room temperature
• 250 mL PureLink Pro 96 Wash Buffer II; room temperature
• 75 mL PureLink Pro 96 RNase-free Water; room temperature
• 4 PureLink 96-well Total RNA Filter Plates; room temperature
• 4 PureLink Pro 96 Elution Plates; room temperature
25 min. (After sample homogenization)
Bacteria, Cells, Plant, Tissue, Yeast
High-throughput Compatible
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Mini
Bacteria: ≤109 cells
Cells: varies
Plant: ≤150 mg
Tissue: ≤100 mg
Yeast: ≤1.8 mL
Silica Plate
45–170 μL
Total RNA
Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis
4 Plates
Room Temperature
Up to 33 μg
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Safety and Handling

Safety and Handling

Product Identifier
  • PureLink Pro 96 total RNA Purification Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 1 B
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P363-Wash contaminated clothing before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Protein denaturant,Thiocyanic acid

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For Research Use Only. Not for use in diagnostic procedures.