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6x-His Tag Monoclonal Antibody (3D5), AP, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen R93225
Additional Details : Weight : 0.00525kg
Description
NOTE: R932-25 was previously sold under product #46-0284. When re-ordering, please use #R932-25. R932-25 detects 6x His tag (C-term) -His-His-His-His-His-His-COOH. Detects fusion proteins containing this (requires the free carboxyl group for detection (Lindner et al., 1997). This product contains enough material for 25 western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, do not dilute the AP-conjugated antibody into PBS. Phosphate inhibits alkaline phosphatase. We recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk. If you use a different buffer for washing and blocking your blots, then dilute as described above with that buffer. You may use other blocking agents such as bovine serum albumin (BSA) or gelatin. All AP-conjugated antibodies have been tested in immunoblotting procedures. Low background was observed. No cross-reactivity has been observed in bacterial lysates.
6x-His Tag Monoclonal antibody specifically detects 6x-His Tag in Tag samples. It is validated for Western Blot.Specifications
| 6x-His Tag | |
| Monoclonal | |
| 1-2 mg/mL | |
| PBS with 0.01% sodium azide | |
| Mouse | |
| purified | |
| RUO | |
| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
| Liquid | |
| Tag |
| Western Blot | |
| 3D5 | |
| Alkaline Phosphatase (AP) | |
| 6His tag; 6X His; His Tag; Histidine Tag | |
| 6x His synthetic peptide: C-term -His-His-His-His-His-His-COOH | |
| 125 μL | |
| Primary | |
| Antibody | |
| IgG2b |