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  7. Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II 

Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

Product Code. 10321035 Shop All Applied Biosystems Products
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Includes:
Buffer I
Buffer II
Quantity:
1,000 units
1000 Units
25,000 units
250 Units
3,000 units
5,000 units
Unit Size:
Each
Product Code. Includes Quantity unitSize
10321035 Buffer II 1000 Units Each
10744427 Buffer II 250 Units Each
10762507 Buffer II 3,000 units Each
10016054 Buffer II 5,000 units Each
10428654 Buffer II 25,000 units Each
10458064 Buffer I 3,000 units Each
10147874 Buffer I 5,000 units Each
10585025 Buffer I 250 Units Each
10468254 Buffer I 1,000 units Each
10516375 Buffer I 25,000 units Each
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Product Code. 10321035

Brand: Applied Biosystems™ N8080172

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Description

Includes

Contains 1000 units of AmpliTaq DNA Polymerase, Buffer II, and MgCl2 Solution.

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.

AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

Reliable and robust PCR

The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.

Features

  • The most thoroughly characterized enzyme available for the PCR process
  • Excellent thermal activity profile makes it good for PCR applications
  • QC-tested to guarantee reproducible results

Notes

  • For superior PCR performance, we recommend DreamTaq DNA Polymerase.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 5 U/μL
Content And Storage • AmpliTaq DNA Polymerase (5 U/μL), 200 μL
• GeneAmp 10X PCR Buffer II, 4 x 1.5 mL
• 25 mM MgCl2, 4 x 1.5 mL

Store at -20°C.
Format Stand-alone enzyme
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 1000 Units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Hot Start No
No. of Reactions 800 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
Includes Buffer II
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For Research Use Only. Not for use in diagnostic procedures.

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