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Thermo Scientific™ BacMam Akt [pSer473] Cellular Assay Kit

Product Code. 10776041
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Product Code. 10776041 Supplier Thermo Scientific™ Supplier No. A12899

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Description

The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.

The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT. BacMam provides a convenient genetic delivery tool for a GFP-AKT fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-AKT at Ser473.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based terbium modification activity (transient), Cell-based terbium phospho-specific activity (transient)
Cell State Cells Not Included
Content And Storage Kit contains Terbium-labeled anti-AKT antibody, BacMam AKT Virus, and 6X Lysis Buffer. Store as indicated in handling documentation.
Description BacMam Akt [pSer473] Cellular Assay Kit, 1 Kit, Fluorescent Detection Method, Kinases Druggable Target, TR-FRET Technique, LanthaScreen™ System, End Point Readout, GFP (EmGFP) Reporter
Detection Method Fluorescence
For Use With (Application) TR-FRET
Human Protein Class Kinases, Cell Signalling
Includes Terbium-labeled anti-AKT antibody, BacMam AKT virus and 6X lysis buffer
Product Line LanthaScreen
Quantity 1 kit
Readout End Point
Reporter Gene GFP (EmGFP)
Target Entry AKT1
Type Cellular Assay Kit
System Type LanthaScreen™
Druggable Target Kinases
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For Research Use Only. Not for use in diagnostic procedures.

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