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Eurofins DiscoverX c-Kit (V654A) Protein, Active

Product Code. 17300634
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Quantity:
10 μg
250 μg
Unit Size:
10µg
250µg
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Product Code. 17300634

Brand: Eurofins DiscoverX 14733

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This item is not returnable. View return policy

Description

Active, N-terminal GST tagged, recombinant human cKit, amino acids 544 - end containing the V654A mutation. V654A is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs, for use in Kinase Assays.

Binding of stem cell factor (SCF) to C-Kit receptor tyrosine kinase induces homodimerization and intermolecular tyrosine phosphorylation of the receptor, creating docking sites for a number of SH2-containing signal transduction molecules. These include phosphatidylinositol 3'-kinase (PI 3K) and phospholipase C-1, Stat1, the Src family kinases Src, Lyn, and Fyn, and the GTPase activating protein GAP. Mutation in Kit which result in ligand-independent activation of the kinase were found to be associated with human gastrointestinal stromal tumors and mastocytosis.

GenBank X06182

Specifications

Formulation mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Molecular Weight (g/mol) 76.7 kDa
Quantity 10 μg
Species Human
Recombinant Recombinant
Protein Tag N-terminal GST
Expression System Recombinant enzyme expressed in Sf21 insect cells
Protein Form Truncated
Purity or Quality Grade ≥77%
Protein Kit (V654A), active
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