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Thermo Scientific™ CaptureSelect™ CH1-XL GoPure™ Column
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CaptureSelect CH1-XL GoPure Columns are ready-to-use columns designed for fast and easy purification of human IgG on standard chromatography systems with high purity and yield in a single step.
The pre-packed column contains CaptureSelect CH1-XL Affinity Matrix, which recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material.
Features of this affinity matrix include:
- Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
- Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
- No co-purification of overproduced free light chains or light chain dimers
- Specific for human antibodies; works in the presence of serum (bovine/horse)
- Non-animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created using a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics:
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect CH1-XL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: >15 mg/mL Fab's depending on flow rate, column height, contact time
Elution conditions: 50 mM sodium acetate buffer, pH 4.0–4.5
Column dimensions: 1 mL column: 7 x 25 mm; 5 mL column: 16 x 25 mm
Max. recommended flow: 1 mL column: 6 mL/min; 5 mL column: 10 mL/min
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol
Specifications
Specifications
| Bed Volume | 1 mL |
| Capacity (Metric) | 1 mL column |
| Matrix | CH1-XL Affinity Matrix |
| Content And Storage | Store at 2°C to 8°C. Protect from direct daylight. |
| Diameter (Metric) | 7 mm |
| Max. Operating Pressure | 0.3 Mpa |
| Particle Size | 63 μm |
| Form | Agarose Bead |
| Product Line | CaptureSelect |
| Quantity | 1 mL |
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For Research Use Only. Not for use in diagnostic procedures.
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