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CD127, eFluor 450, clone: A7R34, eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 48-1271-80

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15361750

  • 132.00€ / 25µg


En stock 3
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Description

Description

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Spécification

Spécification

CD127
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P16872
CD127, IL-7Ralpha, MGC107557
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
A7R34
eFluor 450
Il7r
Liquid
Il7r
IgG2a, kappa, kappa
25μg
4° C, store in dark, DO NOT FREEZE!
Primary
16197
Vidéos

For Research Use Only.