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CD154 (CD40 Ligand), PerCP-eFluor™ 710, clone: MR1, eBioscience™

Armenian Hamster Monoclonal Antibody

Marque:  Affymetrix eBioscience 46-1541-82

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15361290

  • 237.00€ / 100µg


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Description

Description

We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
Spécification

Spécification

CD154 (CD40 Ligand)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P27548
RP23-153G22.3, CD154, CD40-L, Cd40l, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, TRAP, Tnfsf5, gp39, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor ligand superfamily member 5
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
MR1
PerCP-eFluor™ 710
Cd40lg
Liquid
Cd40lg
IgG
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
21947
Vidéos

For Research Use Only.