CD207 (Langerin) Rat anti-Mouse, Alexa Fluor® 488, Clone: eBioRMUL.2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 53-2073-80
Additional Details : Weight : 0.25000kg
Description2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBioRMUL.2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse CD207-transfected HEK-293T cells. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Alexa Fluor™ 488|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.