CD227 (Mucin 1), Alexa Fluor 488, clone: SM3, eBioscience™
Mouse Monoclonal Antibody
Marque: Affymetrix eBioscience 53-9893-80
Informations supplémentaires : Poids : 0.25000kg
DescriptionDescription: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer.Please note this antibody sees a distinct epitope from other Mucin 1 antibodies.Applications Reported: This SM3 antibody has been reported for use in flow cytometric analysis and immunocytochemistry.Applications Tested: This SM3 antibody has been tested by flow cytometry and can be used at less than or equal to 1 μg/mL. This SM3 antibody has also been tested by immunocytochemistry on methanol fixed MCF7 cells and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Epithelial Membrane Antigen, EMA, CD227, Episialin, CD227, EMA, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM, DF3 antigen, H23 antigen, breast carcinoma-associated antigen DF3|
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.