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CD24, eFluor 450, clone: M1/69, eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 48-0242-82

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15361700

  • 198.00€ / 100µg


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Description

Description

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.Applications Reported: This M1/69 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Spécification

Spécification

CD24
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P24807
CD24 antigen (small cell lung carcinoma cluster 4 antigen), CD24A, FLJ22950, FLJ43543, MGC75043
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
M1/69
eFluor 450
Cd24a
Conjugated
Cd24a
IgG2b, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12484
Vidéos

For Research Use Only.