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CD25, Alexa Fluor 488, clone: eBio3C7 (3C7), eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 53-0253-82

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15361990

  • 173.00€ / 100µg


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Description

Description

Description: The eBio3C7 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.Applications Reported: This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio3C7 (3C7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Spécification

Spécification

CD25
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01590
IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, TAC antigen antibody, IL-2R alpha chain
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBio3C7 (3C7)
Alexa Fluor 488
Il2ra
Liquid
Il2ra
IgG2b, kappa, kappa
100μg
4° C, store in dark, DO NOT FREEZE!
Primary
16184
Vidéos

For Research Use Only.