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IBA Lifesciences CD4+ CD25+ Treg Fab Streptamer™ Isolation Kit MB
CD4+ CD25+ Treg Fab Streptamer Isolation Kit MB is for reversible cell isolation delivering label-free target cells.
Brand: IBA Lifesciences 6-8000-205
Additional Details : Weight : 0.01000kg
Description
- Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 3 vials for 5 x 108 cells each
- CD4 Fab-Strep, human for isolation of T-cells; 1 vial for 1 x 109 cells
- CD25 Fab-Strep, human for isolation of T-cells; 1 vial for 5 x 108 cells
- Biotin stock solution for Streptamer™ technology; 3 x 1mL
- Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 2 x 50mL
- Isolation of human CD4+ CD25+ regulatory T cells is performed by serial double positive selection.
- In a first step PBMCs are labeled with magnetic CD4 Fab Streptamers™.
- Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet.
- This CD4 positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).
- The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant.
- Biotin is removed by washing.The pure and label-free CD4 positive cells are subjected to a second positive selection with magnetic CD25 Fab Streptamers™ following the same procedure.
- The resulting cell preparation is highly enriched with CD4+ CD25+ regulatory T cells with a purity of more than 90%.
- No columns are needed.
Specifications
| Cell Isolation Kit | |
| PBMCs |