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CD4, Alexa Fluor 700, clone: GK1.5, eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 56-0041-82

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15362100

  • 207.00€ / 100µg


En stock 2
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Description

Description

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Spécification

Spécification

CD4
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06332
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
GK1.5
Alexa Fluor 700
Cd4
Conjugated
Cd4
IgG2b, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12504
Vidéos

For Research Use Only.