missing translation for 'onlineSavingsMsg'
Learn More

CD45.1 Monoclonal Antibody (A20), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

56.00€ - 253.00€

Specifications

Antigen CD45.1
Clone A20
Concentration 0.5 mg/mL
Applications Flow Cytometry, Immunoprecipitation
Classification Monoclonal
View More Specs

Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15286967
View Documents Promotion Details
Affymetrix eBioscience
14-0453-82
100 μg
94.75€
100µg
View alternative products
Save Up to 
»
Estimated Shipment: 06-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15296967
View Documents Promotion Details
Affymetrix eBioscience
14-0453-85
500 μg
253.00€
500µg
View alternative products
Save Up to 
»
Estimated Shipment: 06-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15276967
View Documents Promotion Details
Affymetrix eBioscience
14-0453-81
50 μg This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
N/A View alternative products
Save Up to 
»
Description

Description

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains. Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Biotinylated A20 is recommended for IHC, cat. 13-0453). Applications Tested: The A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Specifications

Specifications

CD45.1
0.5 mg/mL
Monoclonal
Liquid
RUO
P06800
Ptprc
Primary
4° C
Ptprc
A20
Flow Cytometry, Immunoprecipitation
Unconjugated
Mouse
Mouse
19264
IgG2a κ
Affinity chromatography
Antibody
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.