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Eurofins DiscoverX Cdk7/Cyclin H/MAT1 (CAK complex) Protein, Active

Product Code. p-7228083
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Quantity:
10 μg
250 μg
Unit Size:
10µg
250µg
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Product Code. 17300224

Brand: Eurofins DiscoverX 14476

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Description

Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

Cyclin-dependent Kinase 7 (cdk7) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.

GenBank X79193 / GenBank U11791 / GenBank X87843

Specifications

Formulation mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Molecular Weight (g/mol) 39.9 kDa (CDK7), 37.8 kDa (Cyclin H) and 62.8 kDa (MAT1)
Quantity 10 μg
Species Human
Recombinant Recombinant
Protein Tag C-terminal 6His / untagged / N-terminal GST
Expression System Recombinant enzyme expressed in Sf21 insect cells
Protein Form Full-length
Purity or Quality Grade Combined ≥54%
Protein CDK7/Cyclin E/MAT1, active
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