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  5. Invitrogen™ Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit

Invitrogen™ Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit

Product Code. 17717443
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Quantity:
5 x 96 reactions
96 reactions
Unit Size:
Pack of 480
Pack of 96
This item is not returnable. View return policy

Product Code. 17717443

Brand: Invitrogen™ 18020DFIVE

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Description

The Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit is a solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG, IgM, and IgA in serum and plasma samples. The kit can be used for both qualitative and quantitative assays.

  • A solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG, IgM, and IgA in serum and plasma samples
  • The steps required for use are simply to pre-dilute the reagents, fill the wells of the KingFisher plastic plates, and push start on the instrument
  • After the run, the sample is transferred to a plate compatible with the absorbance reader and the OD is read at 450 nm
  • A DynaMag-96 well magnet can be used while still taking advantage of the faster binding kinetics of magnetic beads compared to the more traditional plate-bound ELISAs
  • Kit contains all buffers required, as well as calibrator antibodies, HRP-conjugated detection antibodies, substrate solution, beads, and stop solution sufficient for one 96-well plate
  • Dynabeads SARS-CoV-2 Spike beads can be purchased separately (18100D) for other uses such as the optimization of unique conditions
  • Using Dynabeads and automation drastically improves reproducibility, limits hands on time, and reduces operating errors
  • The intra-assay CV value is typically <5% (range 2.8–3.2%), while the inter-assay CV is <15% (range 3.9–9.3%)
  • Kit can be used for both qualitative and quantitative assays
  • One of the fastest ELISAs for SARS-CoV-2 serology
  • For research use only, not for use in diagnostic procedures
TRUSTED_SUSTAINABILITY

Specifications

Certifications/Compliance ISO 9001
Content And Storage Kit contains 5X of the following reagents:
• Dynabeads SARS-CoV-2 Spike, 3 mL (1 mg/mL)
• Human SARS-CoV-2 total Ig High Control antibody, 1 vial (lyophilized)
• Human SARS-CoV-2 Total Ig Low Control antibody, 1 vial (lyophilized)
• Human SARS-CoV-2 Total Ig Calibrator (medium control) antibody, 1 vial (lyophilized)
• Wash buffer (20X concentrated), 50 mL
• Assay buffer (20X concentrated), 50 mL
• Substrate solution (tetramethylbenzidine), 15 mL
• Stop solution, 15 mL
• Human SARS-CoV-2 Total Ig HRP Conjugate, 0.12 mL

Store at 2–8°C.
Detection Method Absorbance at 450 nm
Product Type SARS-CoV-2 ELISA Ig Total Kit
For Use With (Application) Binding of human immunoglobulins (IgG, IgM, IgA) towards SARS-CoV-2 (Spike trimer)
For Use With (Equipment) KingFisher™ Flex or Apex Instruments, DynaMag™-96 Side Skirted, Magnetic 96-Well Separator, ELISA Plate Reader (450 nm)
Quantity 5 x 96 reactions
Shipping Condition Wet ice (2° to 8°C)
Diameter (Metric) Bead 2.7 μm
Material Monosized, superparamagnetic polystyrene magnetic beads
High-throughput Compatibility Yes, on KingFisher™ Flex or Apex instruments
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For research use only. Not for use in diagnostic procedures.

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