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IBA Lifesciences pASG-IBA E. coli Expression Vector with Secretion
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Beschreibung
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The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
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The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
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The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
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Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Spezifikation
Spezifikation
| Inducing Agent | Inducible with anhydrotetracycline |
| Marker Gene | Ampicillin |
| Promoter | Tet |
| Product Type | Vector |
| Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | Strep-tag II |
| Quantity | 5 μg |
| Vector | pASG-IBA4 |
| For Use With (Application) | ompA for periplasmic secretion |
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