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Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits
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Quantity:
100 Tests
20 Tests
200 Test
200 Tests
300 Tests
50 Tests
Excitation/Emission:
405, 546/450, 647
488, 535/520, 617
650, 535/660, 617
NA, 535/NA, 617
Conjugate:
APC, Propidium Iodide
Biotin, Propidium Iodide
FITC, Propidium Iodide
PCP-eFluor 710
PE, 7-AAD
PE/Cy7
eFluor 450, 7-AAD
Unit Size:
100 tests
20 tests
200 tests
300 tests
50 tests
Each
Description
Includes
- Annexin V-Biotin protein
- Binding Buffer (4x)
- Propidium Iodide (20 ug/mL)
Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.
Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.
In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).
Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.
In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).
Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.
Specifications
Specifications
| Description | eBioscience Annexin V-Biotin Apoptosis Detection Kit |
| Quantity | 20 Tests |
| Kit Contents | Contains: • Binding Buffer (4X), 1 bottle (50 mL) • rh Annexin V-Biotin, 1 vial (0.1 mL) • Propidium Iodide (20 μg/mL), 1 vial (1.8 mL) |
| Product Type | Apoptosis Detection Kit |
| Content And Storage | Handling Conditions: Store at 2-8°C. Avoid temperature fluctuations. |
| Excitation/Emission | NA, 535/NA, 617 |
| Shipping Condition | Wet Ice |
| Conjugate | Biotin, Propidium Iodide |
| For Use With (Equipment) | Flow Cytometer |
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