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Ephrin B1 Monoclonal Antibody (25H11), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5300-82

Additional Details : Weight : 0.01000kg

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Product Code. 15277407

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.


Description

Description

Description: The monoclonal antibody 25H11 recognizes mouse Ephrin B1, a 47 kDa membrane protein. Ephrin B1 belongs to a family of ephrins composed of type A (linked to the cell membrane through a glycosylphosphatidyl inositol group) or type B (transmembrane) proteins. The type B ephrins consist of 3 members (B1, B2, and B3) each of which contains a cytoplasmic domain containing tyrosine residues that can be phosphorylated. Ephrin B and ephrin B receptors (Eph B) can interact to potentiate bidirectional downstream signaling between cells expressing either or both the ligand and receptor. Ephrins have been shown to play a role in neural crest cell development through both neurogenesis and neural migration, as well as development of kidney and heart cyto-architecture. Overexpression of type B ephrins in cancer cells is associated with increased vascularity and highly invasive tumors. Applications Reported: This 25H11 antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunoprecipitation. Applications Tested: This 25H11 antibody has been tested immunohistochemistry on frozen mouse kidney tissue sections at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Description: The monoclonal antibody 25H11 recognizes mouse Ephrin B1, a 47kDa membrane protein. Ephrin B1 belongs to a family of ephrins composed of type A (linked to the cell membrane through a glycosylphosphatidyl inositol group) or type B (transmembrane) proteins. The type B ephrins consist of 3 members (B1, B2, and B3) each of which contains a cytoplasmic domain containing tyrosine residues that can be phosphorylated. Ephrin B and ephrin B receptors (Eph B) can interact to potentiate bidirectional downstream signaling between cells expressing either or both the ligand and receptor. Ephrins have been shown to play a role in neural crest cell development through both neurogenesis and neural migration, as well as development of kidney and heart cyto-architecture. Overexpression of type B ephrins in cancer cells is associated with increased vascularity and highly invasive tumors.Applications Reported: This 25H11 antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunoprecipitation.Applications Tested: This 25H11 antibody has been tested immunohistochemistry on frozen mouse kidney tissue sections at less than or equal to 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ephrin B1
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q8CBF3
Ephb1
Affinity chromatography
RUO
270190
Store at 2-8°C.
Liquid
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation
25H11
Unconjugated
Ephb1
ELK, EPHT2, FLJ37986, Hek6, NET
Rat
100 μg
Primary
Mouse
Antibody
IgG2a κ
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For Research Use Only.