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Thermo Scientific™ Equine Genotypes Panel 1.1 Kit

Equine Genotypes Panel 1.1 Kit

Brand:  Thermo Scientific™ F850L

Additional Details : Weight : 0.25000kg

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Product Code. 15411134

  • 5900.00€ / 1 set


In stock, ready to ship: 7
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Description

Description

Thermo Scientific Equine Genotypes Panel 1.1 is a reagent kit for horse parentage testing and identification using short tandem repeats (STRs).

The Equine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 17 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Equine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Equine Genotypes Panel 1.1. kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.

The Equine Genotypes Panel 1.1 allows co-amplification of the 17 microsatellites in a single multiplex PCR reaction. The Panel encompasses the following loci: VHL20, HTG4, AHT4, HMS7, HTG6, AHT5, HMS6, ASB23, ASB2, HTG10, HTG7, HMS3, HMS2, ASB17, LEX3, HMS1 and CA425. These include the nine loci recommended by the Equine Genetics and Thoroughbred Parentage Testing Standardization Committee of the International Society for Animal Genetics (ISAG) and eight additional loci commonly used for horse parentage testing and identification.

Highlights

  • Single multiplex PCR of 17 STR loci
  • Master mix and Primer mix provided for easy pipetting
  • Control DNA provided for verification of PCR and electrophoresis conditions
  • Allele calls represents true alleles (no plus-A peaks)
  • Specifications

    Specifications

    Equine Genotypes Panel 1.1 Kit, 500 rxns, Capillary Electrophoresis Detection Method, STR (Short Tandem Repeat) Analysis Technique, Master Mix And Primer Mix Provided For Easy Pipetting, For Animal Health, PCR
    500 rxn(s)
    –20°C
    Capillary electrophoresis
    500 rxn(s)
    5 x 1.1mL Equine genotypes master mix, 5 x 1.1mL equine genotypes panel 1.1 primer mix, 150μL equine genotypes control DNA001
    500 rxn(s)
    Equine genotyping and parentage testing (genotyping)
    Animal health, PCR
    Equine Genotypes Panel 1.1 Kit
    Videos
    SDS
    Documents

    For Research Use Only. Not for use in diagnostic procedures.