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Invitrogen™ Human FGF4 (Cell Lysates) ELISA Kit

Product Code. 17747923
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Quantity:
10 x 96 Tests
Unit Size:
Pack of 10
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17747923 10 x 96 Tests Pack of 10
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Product Code. 17747923 Supplier Invitrogen™ Supplier No. EHFGF4CLX10

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Description

ELISA

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
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Specifications

Accession Number P08620
Assay Range 24.69 to 18,000 pg/mL
Assay Sensitivity 50 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2249
Gene Symbol Fgf4
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Target FGF4
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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