missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. Invitrogen™ Rat Fractalkine-Cell Lysates (CX3CL1) ELISA Kit

Invitrogen™ Rat Fractalkine-Cell Lysates (CX3CL1) ELISA Kit

Product Code. 15445373
Click to view available options
Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
This item is not returnable. View return policy

Product Code. 15445373

Brand: Invitrogen™ ERCXCL1CL

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

ELISA

The Rat Fractalkine-Cell Lysate (CX3CL1) ELISA quantitates Rt Fractalkine in rat cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Rt Fractalkine. Principle of the method The Rat Fractalkine solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O55145
Assay Range 2.74 to 2000 pg/mL
Assay Sensitivity 5 pg/mL
Calibration Range 5 pg/mL to 2000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Chemokine & Receptors
Sample Type Cell Lysates
Specificity The antibody pair provided in this kit recognizes rat Fractalkine.
Sufficient For 96 Assays
Target Species Rat
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 89808
Gene Symbol Cx3c,Scyd1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Oncology
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym Cx3c, Scyd1
Target CX3CL1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
Show More Show Less
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.