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GARP Rat anti-Mouse, PerCP-eFluor™ 710, Clone: YGIC86, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9891-80

Additional Details : Poids : 0.25000kg

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Product Code. 15381400

  • 137.00€ / 25µg


Expédition estimée
02-11-2020
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Description

Description

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.
Specifications

Specifications

GARP
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Liquid
Lrrc32
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
434215
Flow Cytometry
YGIC86
PerCP-eFluor™ 710
Lrrc32
D11S833E, GARP, garpin, glycoprotein A repetitions predomit, leucine-rich repeat-containing protein 32
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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