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Invitrogen™ Gateway™ pENTR™ 4 Dual Selection Vector
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Quantity:
10 μg
Unit Size:
Each
Description
Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone.
The Gateway™ entry vectors (Table 1) offer the following:
- attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
- Kozak consensus sequence for efficient translation initiation in eukaryotic systems
- Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
- rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
- pUC origin for high-copy replication and maintenance of the plasmid in E. coli
- Kanamycin resistance gene for selection in E. coli
- The ccdB/chloramphenicol fusion gene located between the two attL sites for
- o negative selection and
- o Chloramphenicol selection in E. coli
- Kanamycin resistance gene for selection in E. coli
Especificaciones
Especificaciones
| Product Type | Dual SelectionExpression Vector |
| Content And Storage | 10 μg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0. Store at -20°C |
| Antibiotic Resistance Bacterial | Chloramphenicol (CmR), Kanamycin (KanR) |
| Cleavage | No Cleavage Site |
| Protein Tag | Untagged |
| Cloning Method | Gateway |
| Quantity | 10 μg |
| Vector | pENTR |
| Product Line | pENTR |
For Research Use Only. Not for use in diagnostic procedures.
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