missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ GeneBLAzer™ ER Alpha DA Assay Kit

GeneBLAzer™ ER Alpha DA Assay Kit

Brand:  Invitrogen™ K1393

Additional Details : Weight : 0.04000kg

Product Code. 10013434

  • 1448.00€ / Each
Estimated Shipment: 31-07-2024
to see stock.
Add to basket

Description

Description

GeneBLAzer™ER alpha DA(Division Arrested) cells and ER alpha-UAS-bla GripTite™ cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ cell line. GeneBLAzer™UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER alpha DA cells and ER alpha-UAS-bla GripTite™cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Specifications

Specifications

Betamethasone, Cortisol, Budesonide
Cell-based beta lactamase reporter gene
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER alpha GripTite™ DA cells (K1391A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
2099
Dexamethasone
BLA (Beta-Lactamase)
Division-Arrested Cells Assay Kit
GeneBLAzer™
Milfepristone, Cortisone, Progesterone
Division-Arrested Cells
Fluorescent
ERα
GeneBLAzer™
ER alpha, ESR1, ERA, NR3A1
293T (HEK)
Nuclear Receptors
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.