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GL7, eFluor 450, clone: GL-7 (GL7), eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 48-5902-82

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15361800

  • 225.00€ / 100µg


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Description

Description

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis.Applications Tested: This GL-7 (GL7) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Spécification

Spécification

GL7
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Ly-77, Ly77, B cell activation marker, GL7 antigen
IgM
100μg
4° C, store in dark, DO NOT FREEZE!
Primary
HumanþMurine
Flow Cytometry
GL-7 (GL7)
eFluor 450
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Vidéos

For Research Use Only.