missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Product Code. 11585015 Shop All Cytiva Products
Change view
Click to view available options
Quantity:
25 mL
100 mL
500 mL
Unit Size:
100mL
25mL
500mL
Product Code. Quantity unitSize
11585015 25 mL 25mL
11595015 100 mL 100mL
11505025 500 mL 500mL
3 options
This item is not returnable. View return policy

Product Code. 11585015

Brand: Cytiva 17513201

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

  • Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
  • Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
  • Binding capacity and high flow properties allow scale-up
  • Mild elution conditions preserve protein antigenicity and function
  • Stable in all commonly used aqueous buffers
  • pH stability: 3 to 12

TRUSTED_SUSTAINABILITY

Specifications

Binding Capacity >10 mg/mL
Description Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
Flow Rate <450 cm/hr.
Kit Contents 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
Matrix Highly cross-linked 4% agarose
pH Range 3 to 12
Support Type 4% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Particle Size 90 μm
Quantity 25 mL
Type Chromatography Media
Ligand Type Glutathione and 10-carbon linker arm
Show More Show Less

Safety and Handling

compliance-icons
Product Identifier
  • Glutathione Sepharose 4 Fast Flow
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.