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MP Biomedicals HCV Blot 3.0 (CE)

Product Code. 30694927 Shop All MP Biomedicals Products
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Quantity:
18 Tests/Kit
36 Tests/Kit
Unit Size:
1 set
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30694927 18 Tests/Kit 1 set
30694942 36 Tests/Kit 1 set
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Product Code. 30694927 Brand MP Biomedicals Brand No. 11130018

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Description

The Blot is a qualitative enzyme immunoassay for the in vitro detection of antibodies to hepatitis C virus (HCV) in human serum or plasma.

  • The nitrocellulose strips are coated with four recombinant HCV proteins from the Capsid, NS3, NS4, and NS5 regions of the HCV genome.
  • The HCV proteins are expressed as GST fusion proteins, so a GST control band is included to indicate reactivity to native GST.
  • The blots also contain an IgG control band and an anti-IgG band. Individual nitrocellulose strips are incubated with diluted serum or plasma specimens and controls.
  • Specific antibodies to HCV, if present in the specimen, will bind to the HCV proteins on the strips.
  • The strips are washed to remove unbound materials and then incubated with affinity purified anti-human IgG conjugated with alkaline phosphatase.
  • The conjugate antibody will bind to any antigen-antibody complexes formed on the blots.
  • Unbound conjugate is removed by washing. A BCIP/NBT substrate is added to visualise reactive protein bands on the blots.
  • The HCV BLOT 3.0 includes structural and non-structural antigens from HCV and is intended as supplemental confirmatory assay for presence of antibodies to HCV.
  • It is intended for use as a more specific supplemental test on specimens found repeatedly reactive using specific procedures such as ELISA.
  • CE-marked.
  • For professional use only.
  • Human serum and plasma.

Specifications

Product Type Western Blot Kit
Detection Method Colorimetric
For Use With (Application) Immunoblot (Western)
Membrane Compatibility Nitrocellulose
Quantity 18 Tests/Kit
Substrate Alkaline Phosphatase
Product Title
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