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Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
191.00€ - 2343.00€
Specifications
Diameter (English) Inner | 0.6 in. |
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Diameter (Metric) Inner | 16 mm |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Flow Rate | <20 mL/min. |
Material | Polypropylene |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
10126414
|
Cytiva
17-5319-01 |
5 x 1 mL |
191.00€
Pack of 5 |
In Stock
Log in to see stock. |
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11595674
|
Cytiva
17-5319-02 |
100 x 1 mL |
This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance. |
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Description
- Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
- Column material: polypropylene
- Bed dimensions: 16 × 25mm
- Flow velocity: <20mL/min.
- Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
- Compatible with wide range of reducing agents, detergents, denaturants, and other additives
- Simple operation with syringe, perstaltic pump, or chromatographic system
- Columns may be connected in series to increase capacity
Medium
- Matrix: 6% cross-linked agarose
- Average particle size: 90μm
- pH stability working range: 3 to 12
Specifications
0.6 in. | |
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). | |
Polypropylene | |
6% Cross-linked Agarose | |
5 bar (0.5 MPa, 70 psi) |
16 mm | |
<20 mL/min. | |
3 to 12 | |
4°C to 30°C, 20% Ethanol | |
Pre-packed Column |