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Invitrogen™ Granzyme B Human ELISA Kit

For the quantitative detection of human Granzyme B.

Brand:  Invitrogen™ BMS2027

Additional Details : Weight : 0.50000kg

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Product Code. 12747676

  • 546.00€ / Each


Estimated Shipment: 30-06-2021
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Granzyme B
Biotin-Conjugate anti-human Granzyme B monoclonal antibody
Streptavidin-HRP
Human Granzyme B Standard lyophilized, 960pg/mL upon reconstitution
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Dilution Buffer Concentrate 5x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
Specifications

Specifications

P10144
ELISA Kit
0.66-480 pg/mL
3002
RUO
2°C to 8°C
2 hr. 45 min.
Granzyme 2, Fragmentin 2, Human lymphocyte protein
8.5%
Human
0.2 pg/mL
Colorimetric Microplate Reader
HRP
Biotin
ELISA
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Serum, 50 μL|Supernatant, 50 μL
1 hr. 20 min.
Human
10.4%
96 Tests
Supernatant
C11,CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT
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For Research Use Only